Type determination by PCR and Dot Blotting of Streptococcus agalactiae 1 Strains from Zimbabwe Revealed Distinctive Features of Surface - Anchored 2 Proteins 3 4
نویسندگان
چکیده
4 Rooyen T. Mavenyengwa 1 , Johan A. Maeland 2* , Sylvester R. Moyo 3 5 6 7 Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe 1 , 8 Department of Laboratory Medicine, Children’s and Women’s Health, St Olav Hospital, 9 Norwegian University of Science and Technology (NTNU) 2 , Department of Life and Consumer 10 Sciences, School of Agriculture and Life Sciences, University of South Africa (UNISA) 3 . 11 12 Corresponding author. Mailing address: Laboratory of Medical Microbiology, St. Olav’s 13 Hospital, University Hospital, N-7006, and Trondheim, Norway. Phone: 00 47 72 55 71 79. Fax: 14 00 47 72 57 64 17. 15 E-mail: [email protected]. 16 17 Running title: Zimbabwean Streptococcus agalactiae, surface-anchored proteins 18 19
منابع مشابه
Distinctive features of surface-anchored proteins of Streptococcus agalactiae strains from Zimbabwe revealed by PCR and dot blotting.
The distribution of capsular polysaccharide (CPS) types and subtypes (serovariants) among 121 group B streptococcus (GBS) strains from Zimbabwe was examined. PCR was used for the detection of both CPS types and the surface-anchored and strain-variable proteins Calpha, Cbeta, Alp1, Alp2, Alp3, R4/Rib, and Alp4. The R3 protein was detected by an antibody-based method using monoclonal anti-R3 anti...
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Background and Aims: Group B streptococcus (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, theref...
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Group B streptococci (GBS) are important human and bovine pathogens which can be classified by a variety of phenotype- and gene-based techniques. The capsular polysaccharide and strain-variable, surface-anchored proteins are particularly important phenotypic markers. In an earlier study, a previously unrecognized protein antigen called Z was described. It was expressed by 27.2% of GBS strains f...
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Group B Streptococcus (GBS) is a major cause of severe systemic and local infections in newborns. This study was performed to evaluate the presence of GBS in pregnant women and their newborns in Tabriz, Iran. Vaginal specimens were collected from 965 women who were candidate for vaginal delivery for bacteriological study of group B streptococci. Several samples from various sites of the newborn...
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تاریخ انتشار 2008